PENGARUH WAKTU EKSTRAKSI TERHADAP TOTAL FENOLAT DAN NILAI IC50 DARI EKSTRAKSI KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)
Abstract
The skin of the red dragon fruit is a waste that is still very rarely used. While the percentage of red dragon fruit skin is 30% to 35% of the weight of the fruit. Dragon fruit peels still contain quite high antioxidant compounds, antioxidant compounds can fight oxidation in the body. Utilization is done on the skin of dragon fruit, one of which is to extract it so that it can be used as a basis for a variety of functional foods that will benefit health. The study aims to determine the effect of extraction time on total phenolics and antioxidant activity (IC50 values) on red dragon fruit peel extracts and also obtain the optimum extraction time to get the highest or best total phenol and IC50 values. Extraction time used consists of 3 levels, namely 24 hours (1 day), 48 hours (2 days) and 72 hours (3 days). The data obtained were analyzed using the Completely Randomized Design which was applied to the observation of total phenolics and IC50 values, if the treatment had a very significant or real effect followed by continued BNJ testing at 1% or 5% level. The results showed that the extraction time had a very significant effect on the total phenolics and IC50 values of dragon fruit peel extract and the extraction time of 3 hours gave the highest or best total phenolics and IC50 values of 78.78 ppm and 115.90 ppm.
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References
Huliselan, Y. M., Max, R. J., Runtuwene & Wewengkang, D. S. 2015. Aktivitas antioksidan ekstrak etanol, etil asetat, dan n-heksan dari daun sesewanua ( Clerodendron squamatum Vahl.). Pharmacon Jurnal Ilmiah Farmasi, 4(3).
Kristanti, Y., Widarta, I. W. R & Permana, I. D. G. M. 2019. Pengaruh waktu ekstraksi dan konsentrasi etanol menggunakan metode Microwave Assisted Extraction (MAE) terhadap aktivitas antioksidan ekstrak rambut jagung (Zea mays L.). Jurnal Ilmu dan Teknologi Pangan. 8(1): 94-103.
Lestari, P. P., Kusrini. D. & Anam, K. 2014. Anthocyanin identification of methanol-hcl extract active fraction in rosella (Hibiscus sabdariffa L.) and its potential as xanthine oxidase inhibitor. Jurnal Sains dan Matematika. 22(3): 72-78.
Molyneux, P. 2004. The use of the stable free radikal diphenyl picrylhydrazyl (DPPH) for estimating antioxidant activity. Journal Science of Technology. 26(2): 211-219.
Munte, L., Runtuwene, M. R & Citraningtyas, G. 2015. Aktivitas antioksidan dari ekstrak daun prasman (Eupatorium triplinerve Vahl.). Pharmacon Jurnal Ilmiah Farmasi. 4(3).
Novita, M., Sulaiman, M. I & Yura, S. 2016. Pengaruh jenis pelarut terhadap aktivitas antioksidan dan kandungan fenol beberapa jenis bayam dan sayuran lain. Jurnal Ilmiah Mahasiswa Pertanian Unsyiah. 1(1).
Santoso, A. F & Fibrianto, K. 2017. Pengaruh ekstrak kulit buah naga merah (Hylocereus polyrhizus) terhadap kualitas sosis ayam: tinjauan pustaka. Jurnal Pangan dan Agroindustri. 5(4): 92-96.
Sari, A. K. & Ayati, R. 2018. Penentuan aktivitas antioksidan ekstrak etanol daun jeruk purut (Citrus Hystrix D.C) dengan metode DPPH (1,1-diphenyl-2-picrylhydrazyl). Journal Current Pharmateutical Science (JCPS). 1(2): 69-74.
Sari, K.. D., Wardhani, D. H & Prasetyaningrum, A. 2013. Kajian isolasi senyawa fenolik rumput laut Euceuma cottonii berbantu gelombang micro dengan variasi suhu dan waktu. Jurnal Teknik Kimia. 3(19): 38-43.
Wahyuni, D. T & Widjanarko, S. B. 2015. Pengaruh jenis pelarut dan lama ekstraksi terhadap ekstrak karotenoid labu kuning dengan metode gelombang ultrasonik. Jurnal Pangan dan Agroindustri. 3(2): 390-401.
Wati, I., Musadi, M. R., Khumaira, N. S & Amelia, A. R. 2017. Pengaruh konsentrasi pelarut, waktu ekstraksi, dan nisbah bahan baku dengan pelarut terhadap ekstraksi kunyit kuning ( Curcuma longa L.). Jurnal ITEKIMA. 2(1).
Widianingsih, M. 2016. Aktivitas antioksidan ekstrak metanol buah naga merah (Hylocereus polyrhizus (F.A.C Weber) Britton & Rose) hasil maserasi dan dipekatkan dengan kering angin. Jurnal Wiyata. 3(2): 146-150.
Wisesa, T. B & Widjanarko, S. B. 2014. Penentuan nilai maksimum proses ekstraksi kulit buah naga merah (Hylocereus polyrhizus). Jurnal Pangan dan Agroindustri. 2(3): 88-97.
Yuswi, N. C. R. 2017. Ekstraksi antioksidan bawang dayak (Eleutherine palmifolia) dengan metode ultrasonic bath (studi jenis pelarut dan lama ekstraksi). Jurnal Pangan dan Agroindustri. 5(1): 71-79.